VitalTest® facilitates a fast and easy to use Sperm Vitality Testing Method.
VitalTest® differentiates between live and dead sperm cells by identifying those with an intact cell membrane.
While using VitalTest® , live cells appear green and dead cells appear red under a fluorescence microscope. For a high-throughput analysis, the results may be measured by a flow cytometer.
For patients with less than about 40% progressively motile spermatozoa, assessing the number of live and dead sperm cells in a semen sample is especially important. This test acts as a check on motility evaluation, since the number of dead cells should not exceed the percentage of immotile sperm cells.
The presence of a large proportion of immotile and non-viable cells – necrozoospermia – may indicate epididymal pathology (Wilton et al., 1988; Correa-Perez et al., 2004), and a high percentage of vital but immotile cells may be indicative of structural defects in the flagellum (Chemes and Rawe, 2003).
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